In this activity, you will design and conduct your own experiment investigating isopod behavior.
Background Information of our Model Organisms
Isopods: Roly-polies (Armadillidium vulgare)
Terrestrial isopods are land-dwelling crustaceans, commonly known as Roly-polies or pill bugs.  Related to lobsters, crabs, and shrimp, terrestrial isopods breathe with gills. Roly-polies are humid retreats during the day. Roly-polies can be found in compost piles, leaf litter, and beneath logs. Roly-polies usually feed on decomposing plant matter.  Plants decompose with the help of bacteria, which thrive in soil above 4.9 pH. Roly-polies need calcium in their diet to build up their hard protective cuticle, and calcium is not abundant in soils with a pH below 5.5. The ideal soil pH conditions for Roly-polies are above 5.5 where calcium is present but below 9, the maximum threshold for soil bacteria.
Available Materials
10 isopods in a petri dish, choice chamber, stopwatch or timer, list of other materials required:
Conditions
	
		
		
	
	
		
			| temperature | (cool vs. warm) | 
		
			| light  | (light vs. dark or light vs. shade) | 
		
			| pH | (neutral pH vs. low pH vs. high pH) | 
		
			| substrate (soil) | (soil vs no soil) | 
		
			| salinity | (saltwater vs. freshwater) | 
		
			| substrate (leaf litter/organic matter) | (leaf litter vs no leaf litter) |